| Product Name |
Recombinant Human Prolyl-4-Hydroxylase Alpha Polypeptide III (P4Ha3) Protein |
| Product Overview |
This recombinant human Prolyl-4-Hydroxylase Alpha Polypeptide III (P4Ha3) protein includes amino acids 227-544aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBSprior to lyophilization. |
| Target Uniprot Id |
Q7Z4N8 |
| Recommended Name |
Prolyl 4-hydroxylase subunit alpha-3 |
| Gene Name |
P4HA3 |
| Synonyms |
Procollagen-Proline,2-Oxoglutarate 4-Dioxygenase(Proline 4-Hydroxylase),Alpha Polypeptide III |
| Species |
Human |
| Predicted Molecular Mass |
40 kDa |
| Expression System |
E.coli |
| Expression Range |
227-544aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
PBS |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Signal Transduction |
| Target Function |
Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. |
| Subcellular Location |
Endoplasmic reticulum lumen. |
| Protein Family |
P4HA family |
| Tissue Specificity |
Highly expressed in placenta, liver and fetal skin. Weakly expressed in fetal epiphyseal cartilage, fetal liver, fibroblast, lung and skeletal muscle. Expressed also in fibrous cap of carotid atherosclerotic lesions. |
