| Description |
Recombinant Human OSMR&GP130 Protein is expressed from HEK293 with His tag and Flag tag at the C-Terminus.It contains Glu28-Met740(OSMR)&Glu23-Glu619(GP130). |
| Purity |
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Accession |
Q99650-1(OSMR)&P40189-1(GP130) |
| Target Symbol |
OSMR&GP130 |
| Species |
Human |
| Expression System |
HEK293 |
| Tag |
C-His, C-Flag |
| Expression Range |
Glu28-Met740(OSMR) & Glu23-Glu619(GP130) |
| Mol. Weight |
The protein has a predicted MW of 82.2 kDa(OSMR) and 68.83 kDa(GP130). Due to glycosylation, the protein migrates to 100-140 kDa based on Tris-Bis PAGE result. |
| Form |
Lyophilized |
| Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Endotoxin |
Less than 1EU per ug by the LAL method. |
| Activity |
Immobilized Human OSMR&GP130, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA. Contact us for detailed testing images. |
| Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipping |
Shipped at ambient temperature. |
| Gene Background |
OSMR&gp130 is a type II receptor for OSM in humans. OSM can alternatively bind to a heterodimer of gp130 and LIF-R or of gp130 and OSMR (OSM receptor). On the basis of in vitro studies, the OSM ligand recruits a broader array of cell signaling pathways than most gp130 cytokines. OSMR and gp130 chains are widely expressed in connective tissue cells and tumor cells. |
