| Product Name |
Recombinant Human OSGEP Protein |
| Product Overview |
This recombinant human OSGEP protein includes amino acids 1-335aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
Q9NPF4 |
| Recommended Name |
tRNA N6-adenosine threonylcarbamoyltransferase |
| Gene Name |
OSGEP |
| Synonyms |
probable tRNA threonylcarbamoyladenosine biosynthesis protein OSGEP, GCPL1, KAE1, OSGEP1, PRSMG1 |
| Species |
Human |
| Predicted Molecular Mass |
38.8 kDa |
| Expression System |
E.coli |
| Expression Range |
1-335aa |
| Tag |
N-6His |
| Purity |
>85% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Others |
| Target Function |
Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. OSGEP likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity. |
| Subcellular Location |
Cytoplasm. Nucleus. |
| Protein Family |
KAE1 / TsaD family |
| Tissue Specificity |
Widely expressed at low level. Expressed in heart, placenta, liver, kidney, lung, brain, skeletal muscle and pancreas. |
