| Product Name |
Recombinant Human Histone-lysine N-methyltransferase NSD3(NSD3) Protein |
| Product Overview |
This recombinant human Histone-lysine N-methyltransferase NSD3(NSD3) protein includes amino acids 1-645 of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBS pH 7.4, 0.01% SKL, 5% Trehalose, 1% Mannitolprior to lyophilization. |
| Target Uniprot Id |
Q9BZ95 |
| Recommended Name |
Histone-lysine N-methyltransferase NSD3 |
| Gene Name |
NSD3 |
| Synonyms |
NSD3, WHSC1L1, DC28； |
| Species |
Human |
| Predicted Molecular Mass |
105kDa |
| Expression System |
E.coli |
| Expression Range |
1-645 |
| Tag |
N-6His |
| Purity |
>85% |
| Formulation |
Lyophilized |
| Buffer |
PBS pH 7.4, 0.01% SKL, 5% Trehalose, 1% Mannitol |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Epigenetics And Nuclear Signaling |
| Target Function |
Histone methyltransferase. Preferentially dimethylates 'Lys-4' and 'Lys-27' of histone H3 forming H3K2me2 and H3K27me2. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression. |
| Subcellular Location |
Nucleus. Chromosome. |
| Protein Family |
Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, SET2 subfamily |
| Associated Diseases |
Defects in NSD3 may be involved in non small cell lung carcinomas (NSCLC). Amplified or overexpressed in NSCLC. |
| Tissue Specificity |
Highly expressed in brain, heart and skeletal muscle. Expressed at lower level in liver and lung. |
