| Product Name |
Recombinant Human NRAS (EDNRB) Protein |
| Product Overview |
This recombinant human NRAS (EDNRB) protein includes amino acids 1-186aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P24530 |
| Recommended Name |
Endothelin receptor type B |
| Gene Name |
EDNRB |
| Synonyms |
ALPS4;CMNS;N-ras;NCMS;NRAS1;NS6 |
| Species |
Human |
| Predicted Molecular Mass |
23 kDa |
| Expression System |
E.coli |
| Expression Range |
1-186aa |
| Tag |
N-His |
| Purity |
>95% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Signal Transduction |
| Target Function |
Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. |
| Subcellular Location |
Cell membrane; Multi-pass membrane protein. |
| Protein Family |
G-protein coupled receptor 1 family, Endothelin receptor subfamily, EDNRB sub-subfamily |
| Associated Diseases |
Waardenburg syndrome 4A (WS4A); Hirschsprung disease 2 (HSCR2); ABCD syndrome (ABCDS) |
| Tissue Specificity |
Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells. |
