| Description |
Recombinant Human NKG2A&CD94 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Arg100-Leu233(NKG2A)&Ser34-Ile179(CD94). |
| Purity |
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Accession |
P26715-1(NKG2A)&&Q13241-1(CD94) |
| Target Symbol |
NKG2A&CD94 |
| Synonyms |
CD159a; NKG2A; NKG2-A; CD94; NKG2A&CD94 |
| Species |
Human |
| Expression System |
HEK293 |
| Tag |
C-mFc |
| Expression Range |
Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94) |
| Mol. Weight |
The protein has a predicted MW of 56.9 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on Tris-Bis PAGE result. |
| Form |
Lyophilized |
| Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Endotoxin |
Less than 1EU per ug by the LAL method. |
| Activity |
Immobilized Human NKG2A&CD94, mFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA (QC Test). The affinity constant of 7.90 nM as determined in SPR assay (Biacore T200). Contact us for detailed testing images. |
| Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipping |
Shipped at ambient temperature. |
| Gene Background |
The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies. |
