| Product Name |
Recombinant Human Neurofilament, Light Polypeptide (NEFL) Protein |
| Product Overview |
This recombinant human Neurofilament, Light Polypeptide (NEFL) protein includes amino acids 2-543aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBSprior to lyophilization. |
| Target Uniprot Id |
P07196 |
| Recommended Name |
Neurofilament light polypeptide |
| Gene Name |
NEFL |
| Synonyms |
CMT1F; CMT2E; NF-L; NF68; NFL; 68 kDa neurofilament protein; Neurofilament triplet L protein |
| Species |
Human |
| Predicted Molecular Mass |
81 kDa |
| Expression System |
E.coli |
| Expression Range |
2-543aa |
| Tag |
N-6His+SUMO |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
PBS |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Others |
| Target Function |
Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks. |
| Subcellular Location |
Cell projection, axon. Cytoplasm, cytoskeleton. |
| Protein Family |
Intermediate filament family |
| Associated Diseases |
Charcot-Marie-Tooth disease 1F (CMT1F); Charcot-Marie-Tooth disease 2E (CMT2E) |
