| Product Name |
Recombinant Human Neurofilament, Heavy Polypeptide (NEFH) Protein |
| Product Overview |
This recombinant human Neurofilament, Heavy Polypeptide (NEFH) protein includes amino acids 671-1020aa of the target gene is expressed in E.coli.The protein is supplied in liquid or lyophilized powder form and formulated in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.prior to lyophilization. |
| Target Uniprot Id |
P12036 |
| Recommended Name |
Neurofilament heavy polypeptide |
| Gene Name |
NEFH |
| Synonyms |
NFH; 200 kDa neurofilament protein; Neurofilament triplet H protein |
| Species |
Human |
| Predicted Molecular Mass |
52.3 kDa |
| Expression System |
E.coli |
| Expression Range |
671-1020aa |
| Tag |
N-6His |
| Purity |
>95% |
| Formulation |
Liquid or Lyophilized powder |
| Buffer |
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Neuroscience |
| Target Function |
Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks. |
| Subcellular Location |
Cytoplasm, cytoskeleton. Cell projection, axon. |
| Protein Family |
Intermediate filament family |
| Associated Diseases |
Amyotrophic lateral sclerosis (ALS); Charcot-Marie-Tooth disease 2CC (CMT2CC) |
