| Product Name |
Recombinant Human N-Methylpurine DNA Glycosylase (MPG) Protein |
| Product Overview |
This recombinant human N-Methylpurine DNA Glycosylase (MPG) protein includes amino acids 70-220and221-296linkedbyapeptideLCQ of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 200mM NaCl, 1mM EDTAprior to lyophilization. |
| Target Uniprot Id |
P29372 |
| Recommended Name |
DNA-3-methyladenine glycosylase |
| Gene Name |
MPG |
| Synonyms |
DNA-3-methyladenine glycosylase, AAG, ADPG, anpg, APNG, CRA36.1, MDG, Mid1, PIG11, PIG16 |
| Species |
Human |
| Predicted Molecular Mass |
30kDa |
| Expression System |
E.coli |
| Expression Range |
70-220and221-296linkedbyapeptideLCQ |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 200mM NaCl, 1mM EDTA |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Epigenetics And Nuclear Signaling |
| Target Function |
Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions. |
| Subcellular Location |
Cytoplasm. Mitochondrion matrix, mitochondrion nucleoid. Nucleus. |
| Protein Family |
DNA glycosylase MPG family |
