| Product Name |
Recombinant Human Methyl CpG Binding Domain 2 (MBD2) Protein |
| Product Overview |
This recombinant human Methyl CpG Binding Domain 2 (MBD2) protein includes amino acids 145-411 of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBS pH 7.4, 0.01% SKL, 5% Trehalose, 1% Mannitolprior to lyophilization. |
| Target Uniprot Id |
Q9UBB5 |
| Recommended Name |
Methyl-CpG-binding domain protein 2 |
| Gene Name |
MBD2 |
| Synonyms |
DMTase; NY-CO-41; Demethylase; Methyl-CpG-binding protein MBD2 |
| Species |
Human |
| Predicted Molecular Mass |
36kDa |
| Expression System |
E.coli |
| Expression Range |
145-411 |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
PBS pH 7.4, 0.01% SKL, 5% Trehalose, 1% Mannitol |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Epigenetics And Nuclear Signaling |
| Target Function |
Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters. |
| Subcellular Location |
Nucleus. Note=Nuclear, in discrete foci. Detected at replication foci in late S phase. |
| Tissue Specificity |
Highly expressed in brain, heart, kidney, stomach, testis and placenta. |
