| Product Name |
Recombinant Human ERK3 / MAPK12 / P38-gamma (MAPK6) Protein |
| Product Overview |
This recombinant human ERK3 / MAPK12 / P38-gamma (MAPK6) protein includes amino acids 1-367aa of the target gene is expressed in Baculovirus-Insect Cells.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
Q16659 |
| Recommended Name |
Mitogen-activated protein kinase 6 |
| Gene Name |
MAPK6 |
| Synonyms |
ERK-6;ERK3;ERK6;MAPK12;P38GAMMA;PRKM12;SAPK-3;SAPK3 |
| Species |
Human |
| Predicted Molecular Mass |
69.8 kDa |
| Expression System |
Insect Cell |
| Expression Range |
1-367aa |
| Tag |
N-His&GST |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Signal Transduction |
| Target Function |
Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle. |
| Subcellular Location |
Cytoplasm. Nucleus. |
| Protein Family |
Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily |
| Tissue Specificity |
Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts. |
