| Description |
Recombinant Human LY75/CD205 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser28-Asp1666. |
| Purity |
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Accession |
O60449 |
| Target Symbol |
LY75/CD205 |
| Synonyms |
Ly-75; DEC-205; CD205; CLEC13B; GP200-MR6 |
| Species |
Human |
| Expression System |
HEK293 |
| Tag |
C-His |
| Expression Range |
Ser28-Asp1666 |
| Mol. Weight |
The protein has a predicted MW of 190.2 kDa. Due to glycosylation, the protein migrates to 230-245 kDa based on Tris-Bis PAGE result. |
| Form |
Lyophilized |
| Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Endotoxin |
Less than 1EU per ug by the LAL method. |
| Activity |
Immobilized Human LY75, His Tag at 0.1ug/ml (100ul/well) on the plate. Dose response curve for Anti-LY75 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. Contact us for detailed testing images. |
| Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipping |
Shipped at ambient temperature. |
| Gene Background |
Lymphocyte antigen 75 (Ly75/CD205) is a surface marker on mitotic germ cells in rainbow trout. In mammals, several cell surface molecular markers have been characterized in order to identify the mitotic germ cells. he expression profile of Ly75 protein was similar to that of the mRNA. Furthermore, identification of various fish homologs of ly75 confirmed that their amino acid sequences are well conserved. Therefore, Ly75 may be appropriate for use as a versatile cell surface marker for mitotic germ cells in fish. |
