| Description |
Recombinant Human LRP-6-Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Ala20-Pro630. |
| Purity |
> 90% as determined by Tris-Bis PAGE |
| Accession |
O75581 |
| Target Symbol |
LRP-6 |
| Synonyms |
LRP-6; DCAD2; FLJ90062; FLJ90421 |
| Species |
Human |
| Expression System |
HEK293 |
| Tag |
C-mFc |
| Expression Range |
Ala20-Pro630 |
| Mol. Weight |
The protein has a predicted MW of 95.3 kDa. Due to glycosylation, the protein migrates to 110-115 kDa based on Tris-Bis PAGE result. |
| Form |
Lyophilized |
| Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Endotoxin |
Less than 1EU per ug by the LAL method. |
| Activity |
Immobilized Biotinylated Human SOST, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 0.17ug/ml determined by ELISA. Contact us for detailed testing images. |
| Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipping |
Shipped at ambient temperature. |
| Gene Background |
Dickkopf-related protein 1 (DKK-1), a ligand for the WNT coreceptors low-density lipoprotein receptor-related proteins 5 and 6 (LRP-5 and LRP-6) and an inhibitor of WNT/β-catenin signaling, effectively inhibits pericyte activation, detachment, and transition to myofibroblasts in vivo in response to kidney injury, resulting in attenuated fibrogenesis, capillary rarefaction, and inflammation.LRP-6 interacts closely with PDGF receptor β and TGF-β receptor 1 at the cell membrane, suggesting that it may have roles in pathways other than WNT/β-catenin. |
