| Product Name |
Recombinant Human Cytokeratin 18 (CK18/KRT18) Protein |
| Product Overview |
This recombinant human Cytokeratin 18 (CK18/KRT18) protein includes amino acids 2-430aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBSprior to lyophilization. |
| Target Uniprot Id |
P05783 |
| Recommended Name |
Keratin, type I cytoskeletal 18 |
| Gene Name |
KRT18 |
| Synonyms |
KRT18; CYK18; K18; Keratin 18; Cell proliferation-inducing gene 46 protein; Keratin, type I cytoskel |
| Species |
Human |
| Predicted Molecular Mass |
52 kDa |
| Expression System |
E.coli |
| Expression Range |
2-430aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
PBS |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Cell Cycle, , Signal Transduction |
| Target Function |
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. |
| Subcellular Location |
Cytoplasm, perinuclear region. Nucleus, nucleolus. |
| Protein Family |
Intermediate filament family |
| Associated Diseases |
Cirrhosis (CIRRH) |
| Tissue Specificity |
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. |
