| Product Name |
Recombinant Human V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN) Protein |
| Product Overview |
This recombinant human V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN) protein includes amino acids 11-262aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P05412 |
| Recommended Name |
Transcription factor Jun |
| Gene Name |
JUN |
| Synonyms |
Transcription factor AP-1, AP-1, AP1, c-Jun, jun proto-oncogene |
| Species |
Human |
| Predicted Molecular Mass |
34 kDa |
| Expression System |
E.coli |
| Expression Range |
11-262aa |
| Tag |
N-6His |
| Purity |
>85% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Cardiovascular |
| Target Function |
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells. Binds to the USP28 promoter in colorectal cancer (CRC) cells. |
| Subcellular Location |
Nucleus. |
| Protein Family |
BZIP family, Jun subfamily |
| Tissue Specificity |
Expressed in the developing and adult prostate and prostate cancer cells. |
