| Product Name |
Recombinant Human Inosine Triphosphatase Pyrophosphatase (ITPA) Protein |
| Product Overview |
This recombinant human Inosine Triphosphatase Pyrophosphatase (ITPA) protein includes amino acids 3-180aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
Q9BY32 |
| Recommended Name |
Inosine triphosphate pyrophosphatase |
| Gene Name |
ITPA |
| Synonyms |
Inosine triphosphate pyrophosphatase, C20orf37, dJ794I6.3, HLC14-06-P, ITPase, My049, OK/SW-cl.9 |
| Species |
Human |
| Predicted Molecular Mass |
27 kDa |
| Expression System |
E.coli |
| Expression Range |
3-180aa |
| Tag |
N-6His |
| Purity |
>85% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Signal Transduction |
| Target Function |
Pyrophosphatase that hydrolyzes the non-canonical purine nucleotides inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triposphate (dHAPTP) and xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions. |
| Subcellular Location |
Cytoplasm. |
| Protein Family |
HAM1 NTPase family |
| Associated Diseases |
Inosine triphosphate pyrophosphohydrolase deficiency (ITPAD); Epileptic encephalopathy, early infantile, 35 (EIEE35) |
| Tissue Specificity |
Ubiquitous. Highly expressed in heart, liver, sex glands, thyroid and adrenal gland. |
