| Description |
Recombinant Human ITK Protein is expressed from Baculovirus-Insect Cells with GST tag at the N-terminus.It contains Arg352-Leu620. |
| Purity |
> 95% as determined by Tris-Bis PAGE |
| Accession |
NP_005537.3 |
| Target Symbol |
ITK |
| Species |
Human |
| Expression System |
Baculovirus-Insect Cells |
| Tag |
N-GST |
| Expression Range |
Arg352-Leu620 |
| Mol. Weight |
The protein has a predicted MW of 57.33 kDa same as Tris-Bis PAGE result. |
| Form |
Liquid |
| Formulation |
Supplied as 0.22 um filtered solution in 50mM Tris, 150mM NaCl, 25% glycerol, 0.5mM PMSF, 0.5 mM TCEP, 0.1mM EDTA, 0.5mM GSH (pH 7.5). |
| Endotoxin |
Less than 1EU per ug by the LAL method. |
| Storage |
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
| Shipping |
Shipped with dry ice. |
| Gene Background |
Itk/Emt, a tec family tyrosine kinase, is important for T-cell development and activation through the antigen receptor. Itk/Emt can induce the generation of important second signals (PKC, Ca2+) and can modulate their duration by maintaining the stability of scaffolding complexes through regulation of cytoskeletal reorganization. |
