| Product Name |
Recombinant Human Immunity-related GTPase family M(IRGM) Protein |
| Product Overview |
This recombinant human Immunity-related GTPase family M(IRGM) protein includes amino acids 23-181aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
A1A4Y4 |
| Recommended Name |
Immunity-related GTPase family M protein |
| Gene Name |
IRGM |
| Synonyms |
Immunity-related GTPase family M protein, IFI1, IRGM1, LRG-47, LRG47, MGC149263, MGC149264 |
| Species |
Human |
| Predicted Molecular Mass |
20.1 kDa |
| Expression System |
E.coli |
| Expression Range |
23-181aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Immunology |
| Target Function |
Putative GTPase which is required for clearance of acute protozoan and bacterial infections. Functions in innate immune response probably through regulation of autophagy. May regulate proinflammatory cytokine production and prevent endotoxemia upon infection. May also play a role in macrophages adhesion and motility. |
| Subcellular Location |
Golgi apparatus membrane. Cell membrane. Cytoplasmic vesicle, phagosome membrane. Cytoplasmic vesicle, autophagosome membrane. Cell projection, phagocytic cup. |
| Protein Family |
TRAFAC class dynamin-like GTPase superfamily, IRG family |
| Associated Diseases |
Inflammatory bowel disease 19 (IBD19) |
| Tissue Specificity |
Widely expressed (at protein level). Expressed in several tissues including colon, small bowel and peripheral blood leukocytes. |
