| Product Name |
Recombinant Human Interleukin 21 (IL21) Protein |
| Product Overview |
This recombinant human Interleukin 21 (IL21) protein includes amino acids 32-162 of the target gene is expressed in HEK293 Cells.The protein is supplied in lyophilized form and formulated in PBS pH 7.4, 0.01% SKL, 5% Trehalose, 1% Mannitolprior to lyophilization. |
| Target Uniprot Id |
Q9HBE4 |
| Recommended Name |
Interleukin-21 |
| Gene Name |
IL21 |
| Synonyms |
CVID11;IL-21;Za11 |
| Species |
Human |
| Predicted Molecular Mass |
19kDa |
| Expression System |
Mammalian Cell |
| Expression Range |
32-162 |
| Tag |
C-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
PBS pH 7.4, 0.01% SKL, 5% Trehalose, 1% Mannitol |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Immunology |
| Target Function |
Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection. May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. |
| Subcellular Location |
Secreted. |
| Protein Family |
IL-15/IL-21 family |
| Associated Diseases |
Immunodeficiency, common variable, 11 (CVID11) |
| Tissue Specificity |
Expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes. |
