| Product Name |
Recombinant Human Interleukin 1 Beta (IL1b) Protein |
| Product Overview |
This recombinant human Interleukin 1 Beta (IL1b) protein includes amino acids 117-269aa of the target gene is expressed in 293F.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P01584 |
| Recommended Name |
Interleukin-1 beta |
| Gene Name |
IL1B |
| Synonyms |
Interleukin 1β, IL-1, IL1F2, IL1-beta, Catabolin |
| Species |
Human |
| Predicted Molecular Mass |
21 kDa |
| Expression System |
Mammalian Cell |
| Expression Range |
117-269aa |
| Tag |
N-6His |
| Purity |
>95% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Immunology |
| Target Function |
Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. |
| Subcellular Location |
Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted. |
| Protein Family |
IL-1 family |
| Tissue Specificity |
Expressed in activated monocytes/macrophages (at protein level). |
