| Description |
Recombinant Human IL-2 R beta&IL-2 R gamma Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ala27-Thr240 (IL-2 R beta)&Leu23-Ala262 (IL-2 R gamma). |
| Purity |
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Accession |
P14784(IL-2 R beta)&P31785-1(IL-2 R gamma) |
| Target Symbol |
IL-2 R beta&IL-2 R gamma |
| Synonyms |
IL-2RB&IL-2RG IL-2RG; IL-2R subunit gamma; IL-2R subunit beta; IL-2RB; IL15RB; IL-2 R beta&IL-2 R gamma |
| Species |
Human |
| Expression System |
HEK293 |
| Tag |
C-hFc |
| Expression Range |
Ala27-Thr240(IL-2 R beta) & Leu23-Ala262(IL-2 R gamma) |
| Mol. Weight |
The protein has a predicted MW of 51.3 kDa (IL-2 R beta)&54.6 kDa (IL-2 R gamma). Due to glycosylation, the protein migrates to 65-70 kDa&80-100 kDa based on Tris-Bis PAGE result. |
| Form |
Lyophilized |
| Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Endotoxin |
Less than 1EU per ug by the LAL method. |
| Activity |
Immobilized Human IL-15RA&IL-15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 38.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.114 nM as determined in SPR assay (Biacore T200). Contact us for detailed testing images. |
| Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipping |
Shipped at ambient temperature. |
| Gene Background |
Wilms' tumor 1 (WT1) is a transcription factor which plays a major role in cell proliferation, differentiation, survival, and apoptosis. WT1 was first identified as a tumor suppressor gene in Wilms' tumor. The expression of survival signaling genes, IL-2, IL-2RB, and IL-2RG, was also suppressed after WT1-siRNA treatment. In addition, the WT1 silencing also inhibited the S phase of the cell cycle and induced cell death.Moreover, transcriptional modulation of IL-2, IL-2RB, and IL2-2RG expression by WT1 was likely involved in this phenotypic change. |
