| Product Name |
Recombinant Human Isoleucine--tRNA ligase, cytoplasmic (IARS/IARS1) Protein |
| Product Overview |
This recombinant human Isoleucine--tRNA ligase, cytoplasmic (IARS/IARS1) protein includes amino acids 693-852aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBSprior to lyophilization. |
| Target Uniprot Id |
P41252 |
| Recommended Name |
Isoleucine--tRNA ligase, cytoplasmic |
| Gene Name |
IARS1 |
| Synonyms |
IARS1; ILRS; OJ; IleRS; IRS; Isoleucine tRNA Ligase 1,Cytoplasmic |
| Species |
Human |
| Predicted Molecular Mass |
24 kDa |
| Expression System |
E.coli |
| Expression Range |
693-852aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
PBS |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Epigenetics And Nuclear Signaling |
| Target Function |
Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. |
| Subcellular Location |
Cytoplasm. Cytoplasm, cytosol. |
| Protein Family |
Class-I aminoacyl-tRNA synthetase family |
| Associated Diseases |
Growth retardation, intellectual developmental disorder, hypotonia, and hepatopathy (GRIDHH) |
| Tissue Specificity |
Expressed in liver and muscle (at protein level). |
