| Product Name |
Recombinant Human 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) Protein |
| Product Overview |
This recombinant human 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) protein includes amino acids 2-292aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBSprior to lyophilization. |
| Target Uniprot Id |
P28845 |
| Recommended Name |
11-beta-hydroxysteroid dehydrogenase 1 |
| Gene Name |
HSD11B1 |
| Synonyms |
11DH; 11-beta-HSD1; HDL; HSD11; HSD11B; HSD11L; SDR26C1 |
| Species |
Human |
| Predicted Molecular Mass |
35 kDa |
| Expression System |
E.coli |
| Expression Range |
2-292aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
PBS |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Target Function |
Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol. |
| Subcellular Location |
Endoplasmic reticulum membrane; Single-pass type II membrane protein. |
| Protein Family |
Short-chain dehydrogenases/reductases (SDR) family |
| Associated Diseases |
Cortisone reductase deficiency 2 (CORTRD2) |
| Tissue Specificity |
Widely expressed. Highest expression in liver. |
