| Product Name |
Recombinant Human 3-Hydroxymethyl-3-Methylglutaryl Coenzyme A Lyase (HMGCL) Protein |
| Product Overview |
This recombinant human 3-Hydroxymethyl-3-Methylglutaryl Coenzyme A Lyase (HMGCL) protein includes amino acids 1-325aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P35914 |
| Recommended Name |
Hydroxymethylglutaryl-CoA lyase, mitochondrial |
| Gene Name |
HMGCL |
| Synonyms |
Hydroxymethylglutaryl-CoA lyase, HL |
| Species |
Human |
| Predicted Molecular Mass |
38 kDa |
| Expression System |
E.coli |
| Expression Range |
1-325aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Signal Transduction |
| Target Function |
Mitochondrial 3-hydroxymethyl-3-methylglutaryl-CoA lyase that catalyzes a cation-dependent cleavage of (S)-3-hydroxy-3-methylglutaryl-CoA into acetyl-CoA and acetoacetate, a key step in ketogenesis. Terminal step in leucine catabolism. Ketone bodies (beta-hydroxybutyrate, acetoacetate and acetone) are essential as an alternative source of energy to glucose, as lipid precursors and as regulators of metabolism. |
| Subcellular Location |
Mitochondrion matrix. Peroxisome. |
| Protein Family |
HMG-CoA lyase family |
| Associated Diseases |
3-hydroxy-3-methylglutaryl-CoA lyase deficiency (HMGCLD) |
| Tissue Specificity |
Highest expression in liver. Expressed in pancreas, kidney, intestine, testis, fibroblasts and lymphoblasts. Very low expression in brain and skeletal muscle. The relative expression of isoform 2 (at mRNA level) is highest in heart (30%), skeletal muscle |
