| Product Name |
Recombinant Human Maleylacetoacetate isomerase(GSTZ1) Protein |
| Product Overview |
This recombinant human Maleylacetoacetate isomerase(GSTZ1) protein includes amino acids 1-216aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
O43708 |
| Recommended Name |
Maleylacetoacetate isomerase |
| Gene Name |
GSTZ1 |
| Synonyms |
Glutathione S-transferase zeta 1, GSTZ1-1, MAAI, MAI |
| Species |
Human |
| Predicted Molecular Mass |
26.2 kDa |
| Expression System |
E.coli |
| Expression Range |
1-216aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Signal Transduction |
| Target Function |
Bifunctional enzyme showing minimal glutathione-conjugating activity with ethacrynic acid and 7-chloro-4-nitrobenz-2-oxa-1,3-diazole and maleylacetoacetate isomerase activity. Has also low glutathione peroxidase activity with T-butyl and cumene hydroperoxides. Is able to catalyze the glutathione dependent oxygenation of dichloroacetic acid to glyoxylic acid. |
| Subcellular Location |
Cytoplasm. |
| Protein Family |
GST superfamily, Zeta family |
| Associated Diseases |
Maleylacetoacetate isomerase deficiency (MAAID) |
| Tissue Specificity |
Mostly expressed in liver followed by kidney, skeletal muscle and brain. Also expressed in melanocytes, synovium, placenta, breast and fetal liver and heart. |
