| Product Name |
Recombinant Human Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2A (GRIN2A) Protein |
| Product Overview |
This recombinant human Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2A (GRIN2A) protein includes amino acids 31-555aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBSprior to lyophilization. |
| Target Uniprot Id |
Q12879 |
| Recommended Name |
Glutamate receptor ionotropic, NMDA 2A |
| Gene Name |
GRIN2A |
| Synonyms |
NMDAR2A; NR2A |
| Species |
Human |
| Predicted Molecular Mass |
63 kDa |
| Expression System |
E.coli |
| Expression Range |
31-555aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
PBS |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Transport |
| Target Function |
Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). Sensitivity to glutamate and channel kinetics depend on the subunit composition; channels containing GRIN1 and GRIN2A have lower sensitivity to glutamate and faster deactivation kinetics than channels formed by GRIN1 and GRIN2B. Contributes to the slow phase of excitatory postsynaptic current, long-term synaptic potentiation, and learning. |
| Subcellular Location |
Cell projection, dendritic spine. Cell membrane; Multi-pass membrane protein. Cell junction, synapse. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane. |
| Protein Family |
Glutamate-gated ion channel (TC 1.A.10.1) family, NR2A/GRIN2A subfamily |
| Associated Diseases |
Epilepsy, focal, with speech disorder and with or without mental retardation (FESD) |
