| Product Name |
Recombinant Human G-protein coupled bile acid receptor 1(GPBAR1) Protein |
| Product Overview |
This recombinant human G-protein coupled bile acid receptor 1(GPBAR1) protein includes amino acids 283-330 of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBS pH 7.4, 0.01% SKL, 5% Trehalose, 1% Mannitolprior to lyophilization. |
| Target Uniprot Id |
Q8TDU6 |
| Recommended Name |
G-protein coupled bile acid receptor 1 |
| Gene Name |
GPBAR1 |
| Synonyms |
GPBAR |
| Species |
Human |
| Predicted Molecular Mass |
21kDa |
| Expression System |
E.coli |
| Expression Range |
283-330 |
| Tag |
N-6His+sumo |
| Purity |
>85% |
| Formulation |
Lyophilized |
| Buffer |
PBS pH 7.4, 0.01% SKL, 5% Trehalose, 1% Mannitol |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Others |
| Target Function |
Receptor for bile acid. Bile acid-binding induces its internalization, activation of extracellular signal-regulated kinase and intracellular cAMP production. May be involved in the suppression of macrophage functions by bile acids. |
| Subcellular Location |
Cell membrane; Multi-pass membrane protein. |
| Protein Family |
G-protein coupled receptor 1 family |
| Tissue Specificity |
Ubiquitously expressed. Expressed at higher level in spleen and placenta. Expressed at lower level in other tissues. In digestive tissues, it is expressed in stomach, duodenum, ileocecum, ileum, jejunum, ascending colon, transverse colon, descending colon |
