| Description |
Recombinant Human GARP&Latent TGF Beta 2 Complex Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His20-Leu628(GARP)&Leu21-Ser414(Latent TGF Beta 2). |
| Purity |
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Accession |
Q14392(GARP)&P61812-1(Latent TGF Beta 2) |
| Target Symbol |
GARP&Latent TGF Beta 2 Complex |
| Species |
Human |
| Expression System |
HEK293 |
| Tag |
C-His-Avi |
| Expression Range |
His20-Leu628(GARP) & Leu21-Ser414(Latent TGF Beta 2) |
| Mol. Weight |
The protein has a predicted MW of 70.3 kDa(GARP)&45.53 kDa(Latent TGF Beta 2). Due to enzyme lysis and glycosylation, the protein migrates to 72-77 kDa(GARP)&13 kDa&46-48 kDa(Latent TGF Beta 2) based on Tris-Bis PAGE result. |
| Form |
Lyophilized |
| Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Endotoxin |
Less than 1EU per ug by the LAL method. |
| Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipping |
Shipped at ambient temperature. |
| Gene Background |
GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex. |
