| Product Name |
Recombinant Human Farnesyl Diphosphate Farnesyltransferase 1 (FDFT1) Protein |
| Product Overview |
This recombinant human Farnesyl Diphosphate Farnesyltransferase 1 (FDFT1) protein includes amino acids 269-417aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P37268 |
| Recommended Name |
Squalene synthase |
| Gene Name |
FDFT1 |
| Synonyms |
Squalene synthase, DGPT, ERG9, SQS, SS |
| Species |
Human |
| Predicted Molecular Mass |
18 kDa |
| Expression System |
E.coli |
| Expression Range |
269-417aa |
| Tag |
N-6His |
| Purity |
>95% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Metabolism |
| Target Function |
Catalyzes the condensation of 2 farnesyl pyrophosphate (FPP) moieties to form squalene. Proceeds in two distinct steps. In the first half-reaction, two molecules of FPP react to form the stable presqualene diphosphate intermediate (PSQPP), with concomitant release of a proton and a molecule of inorganic diphosphate. In the second half-reaction, PSQPP undergoes heterolysis, isomerization, and reduction with NADPH or NADH to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway. |
| Subcellular Location |
Endoplasmic reticulum membrane; Multi-pass membrane protein. |
| Protein Family |
Phytoene/squalene synthase family |
| Tissue Specificity |
Widely expressed. |
