| Product Name |
Recombinant Human Coagulation Factor XII (F12) Protein |
| Product Overview |
This recombinant human Coagulation Factor XII (F12) protein includes amino acids 400-615aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBSprior to lyophilization. |
| Target Uniprot Id |
P00748 |
| Recommended Name |
Coagulation factor XII |
| Gene Name |
F12 |
| Synonyms |
FXII; HAF; Contact Factor; Hageman Factor |
| Species |
Human |
| Predicted Molecular Mass |
28 kDa |
| Expression System |
E.coli |
| Expression Range |
400-615aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
PBS |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Cardiovascular |
| Target Function |
Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa. |
| Subcellular Location |
Secreted. |
| Protein Family |
Peptidase S1 family |
| Associated Diseases |
Factor XII deficiency (FA12D); Hereditary angioedema 3 (HAE3) |
