| Product Name |
Recombinant Human Persulfide dioxygenase ETHE1, mitochondrial (ETHE1) Protein |
| Product Overview |
This recombinant human Persulfide dioxygenase ETHE1, mitochondrial (ETHE1) protein includes amino acids 13-254aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
O95571 |
| Recommended Name |
Persulfide dioxygenase ETHE1, mitochondrial |
| Gene Name |
ETHE1 |
| Synonyms |
Protein ETHE1, mitochondrial. |
| Species |
Human |
| Predicted Molecular Mass |
29.1 kDa |
| Expression System |
E.coli |
| Expression Range |
13-254aa |
| Tag |
N-6His |
| Purity |
>95% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Cell Biology |
| Target Function |
Sulfur dioxygenase that plays an essential role in hydrogen sulfide catabolism in the mitochondrial matrix. Hydrogen sulfide (H(2)S) is first oxidized by SQRDL, giving rise to cysteine persulfide residues. ETHE1 consumes molecular oxygen to catalyze the oxidation of the persulfide, once it has been transferred to a thiophilic acceptor, such as glutathione (R-SSH). Plays an important role in metabolic homeostasis in mitochondria by metabolizing hydrogen sulfide and preventing the accumulation of supraphysiological H(2)S levels that have toxic effects, due to the inhibition of cytochrome c oxidase. First described as a protein that can shuttle between the nucleus and the cytoplasm and suppress p53-induced apoptosis by sequestering the transcription factor RELA/NFKB3 in the cytoplasm and preventing its accumulation in the nucleus. |
| Subcellular Location |
Cytoplasm. Nucleus. Mitochondrion matrix. |
| Protein Family |
Metallo-beta-lactamase superfamily, Glyoxalase II family |
| Associated Diseases |
Ethylmalonic encephalopathy (EE) |
| Tissue Specificity |
Ubiquitously expressed. |
