| Product Name |
Recombinant Human Epithelial Cell Adhesion Molecule (EPCAM) Protein |
| Product Overview |
This recombinant human Epithelial Cell Adhesion Molecule (EPCAM) protein includes amino acids 24-264aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P16422 |
| Recommended Name |
Epithelial cell adhesion molecule |
| Gene Name |
EPCAM |
| Synonyms |
Epithelial cell adhesion molecules, CD326, KS1/4, KSA, M4S1, MIC18, MK-1, TACSTD1, TROP1, DIAR5, EGP |
| Species |
Human |
| Predicted Molecular Mass |
62 kDa |
| Expression System |
E.coli |
| Expression Range |
24-264aa |
| Tag |
N-6His+GST |
| Purity |
>85% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Tags & Cell Markers |
| Target Function |
May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. |
| Subcellular Location |
Lateral cell membrane; Single-pass type I membrane protein. Cell junction, tight junction. |
| Protein Family |
EPCAM family |
| Associated Diseases |
Diarrhea 5, with tufting enteropathy, congenital (DIAR5); Hereditary non-polyposis colorectal cancer 8 (HNPCC8) |
| Tissue Specificity |
Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal |
