| Product Name |
Recombinant Human Endothelin 1 (EDN1) Protein |
| Product Overview |
This recombinant human Endothelin 1 (EDN1) protein includes amino acids 53-90aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBSprior to lyophilization. |
| Target Uniprot Id |
P05305 |
| Recommended Name |
Endothelin-1 |
| Gene Name |
EDN1 |
| Synonyms |
ET1; PPET1; Preproendothelin-1; Big endothelin-1 |
| Species |
Human |
| Predicted Molecular Mass |
6 kDa |
| Expression System |
E.coli |
| Expression Range |
53-90aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
PBS |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Signal Transduction |
| Target Function |
Endothelins are endothelium-derived vasoconstrictor peptides. Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells. |
| Subcellular Location |
Secreted. |
| Protein Family |
Endothelin/sarafotoxin family |
| Associated Diseases |
Question mark ears, isolated (QME); Auriculocondylar syndrome 3 (ARCND3) |
| Tissue Specificity |
Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. |
