| Product Name |
Recombinant Human Endostatin (ES/COL18A1) Protein |
| Product Overview |
This recombinant human Endostatin (ES/COL18A1) protein includes amino acids 1154-1335aa of the target gene is expressed in 293F cell.The protein is supplied in lyophilized form and formulated in PBSprior to lyophilization. |
| Target Uniprot Id |
P39060 |
| Recommended Name |
Collagen alpha-1(XVIII) chain |
| Gene Name |
COL18A1 |
| Synonyms |
COL18A1,Collagen alpha-1(XVIII) chain |
| Species |
Human |
| Predicted Molecular Mass |
21 kDa |
| Expression System |
Mammalian Cell |
| Expression Range |
1154-1335aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
PBS |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Cell Adhesion |
| Target Function |
Probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.; May regulate extracellular matrix-dependent motility and morphogenesis of endothelial and non-endothelial cells; the function requires homotrimerization and implicates MAPK signaling.; Potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling. Inhibits VEGFA-induced endothelial cell proliferation and migration. Seems to inhibit VEGFA-mediated signaling by blocking the interaction of VEGFA to its receptor KDR/VEGFR2. Modulates endothelial cell migration in an integrin-dependent manner implicating integrin ITGA5:ITGB1 and to a lesser extent ITGAV:ITGB3 and ITGAV:ITGB5. May negatively regulate the activity of homotrimeric non-collagenous domain 1. |
| Subcellular Location |
Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, extracellular matrix, basement membrane.; [Non-collagenous domain 1]: Secreted, extracellular space, extracellular matrix, basement membrane. Secreted.; [Endostatin]: Secreted. Secreted, extracellular space, extracellular matrix, basement membrane. |
| Protein Family |
Multiplexin collagen family |
| Associated Diseases |
Knobloch syndrome 1 (KNO1) |
| Tissue Specificity |
Present in multiple organs with highest levels in liver, lung and kidney. |
