| Product Name |
Recombinant Human Neutrophil Elastase (NE/ELANE) Protein |
| Product Overview |
This recombinant human Neutrophil Elastase (NE/ELANE) protein includes amino acids 30-247aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBSprior to lyophilization. |
| Target Uniprot Id |
P08246 |
| Recommended Name |
Neutrophil elastase |
| Gene Name |
ELANE |
| Synonyms |
ELANE; HLE; HNE; ELA2; PMN-E; Neutrophil Elastase; Medullasin; Polymorphonuclear Leukocyte Elastase; |
| Species |
Human |
| Predicted Molecular Mass |
53 kDa |
| Expression System |
E.coli |
| Expression Range |
30-247aa |
| Tag |
N-His-GST |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
PBS |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Immunology, Signal Transduction |
| Target Function |
Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae. |
| Subcellular Location |
Cytoplasmic vesicle, phagosome. |
| Protein Family |
Peptidase S1 family, Elastase subfamily |
| Associated Diseases |
Cyclic haematopoiesis (CH); Neutropenia, severe congenital 1, autosomal dominant (SCN1) |
| Tissue Specificity |
Bone marrow cells. Neutrophil. |
