| Product Name |
Recombinant Human EEF1A1 Protein |
| Product Overview |
This recombinant human EEF1A1 protein includes amino acids 1-462aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in 20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerolprior to lyophilization. |
| Target Uniprot Id |
P68104 |
| Recommended Name |
Elongation factor 1-alpha 1 |
| Gene Name |
EEF1A1 |
| Synonyms |
Eukaryotic translation elongation factor 1 alpha 1, CCS-3, CCS3, EEF-1, EEF1A, eEF1A-1, EF-Tu, EF1A, FLJ25721, GRAF-1EF, HNGC:16303, PTI1 |
| Species |
Human |
| Predicted Molecular Mass |
50 kDa (462aa) |
| Expression System |
E.coli |
| Expression Range |
1-462aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
20mM Tris-HCl buffer (pH 8.0) containing 0.4M Urea, 10% glycerol |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Epigenetics And Nuclear Signaling |
| Target Function |
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1.; (Microbial infection) Required for the translation of viral proteins and viral replication during human coronavirus SARS-CoV-2 infection. |
| Subcellular Location |
Cytoplasm. Nucleus. Nucleus, nucleolus. Cell membrane. |
| Protein Family |
TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, EF-Tu/EF-1A subfamily |
| Tissue Specificity |
Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle. |
