| Product Name |
Recombinant Human DPH2 Protein |
| Product Overview |
This recombinant human DPH2 protein includes amino acids 1-489aa of the target gene is expressed in Baculovirus-Insect Cells.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
Q9BQC3 |
| Recommended Name |
2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 |
| Gene Name |
DPH2 |
| Synonyms |
Diphthamide biosynthesis protein 2, DPH2, DPH2L2 |
| Species |
Human |
| Predicted Molecular Mass |
53.1 kDa |
| Expression System |
Insect Cell |
| Expression Range |
1-489aa |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Cell Biology |
| Target Function |
Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2). |
| Protein Family |
DPH1/DPH2 family, DPH2 subfamily |
| Tissue Specificity |
Strongly expressed in skeletal muscle. Moderately expressed in heart, small intestine, liver, pancreas, testis and colon. Weakly expressed in brain, placenta, kidney, spleen, thymus, prostate, ovary and lymphocytes. |
