| Product Name |
Recombinant Human Heat Shock 40kDa 3 (HSPF3/DNAJB2) Protein |
| Product Overview |
This recombinant human Heat Shock 40kDa 3 (HSPF3/DNAJB2) protein includes amino acids 1-277aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P25686 |
| Recommended Name |
DnaJ homolog subfamily B member 2 |
| Gene Name |
DNAJB2 |
| Synonyms |
DnaJ homolog subfamily B member 2, HSJ1, HSPF3. |
| Species |
Human |
| Predicted Molecular Mass |
37 kDa |
| Expression System |
E.coli |
| Expression Range |
1-277aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Signal Transduction |
| Target Function |
Functions as a co-chaperone, regulating the substrate binding and activating the ATPase activity of chaperones of the HSP70/heat shock protein 70 family. In parallel, also contributes to the ubiquitin-dependent proteasomal degradation of misfolded proteins. Thereby, may regulate the aggregation and promote the functional recovery of misfolded proteins like HTT, MC4R, PRKN, RHO and SOD1 and be crucial for many biological processes. Isoform 1 which is localized to the endoplasmic reticulum membranes may specifically function in ER-associated protein degradation of misfolded proteins. |
| Subcellular Location |
[Isoform 2]: Cytoplasm. Nucleus.; [Isoform 1]: Endoplasmic reticulum membrane; Lipid-anchor; Cytoplasmic side. |
| Associated Diseases |
Distal spinal muscular atrophy, autosomal recessive, 5 (DSMA5) |
| Tissue Specificity |
More abundantly expressed in neocortex, cerebellum, spinal cord and retina where it is expressed by neuronal cells (at protein level). Detected at much lower level in non-neuronal tissues including kidney, lung, heart, skeletal muscle, spleen and testis ( |
