| Description |
Recombinant Human DLL4 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ser27-Pro524. |
| Purity |
> 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC |
| Accession |
KT340 |
| Target Symbol |
DLL4 |
| Species |
Human |
| Expression System |
HEK293 |
| Tag |
C-His |
| Expression Range |
Ser27-Pro524 |
| Mol. Weight |
The protein has a predicted MW of 55.38 kDa. Due to glycosylation, the protein migrates to 56-70 kDa based on Tris-Bis PAGE result. |
| Form |
Lyophilized |
| Formulation |
Lyophilized from 0.22 um filtered solution in PBS, 200 mM L-Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Endotoxin |
Less than 1EU per ug by the LAL method. |
| Activity |
Immobilized Human DLL4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DLL4 Antibody, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA. Contact us for detailed testing images. |
| Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Shipping |
Shipped at ambient temperature. |
| Gene Background |
Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (By similarity). During spinal cord neurogenesis, inhibits V2a interneuron fate. |
