| Product Name |
Recombinant Human DHFR Protein |
| Product Overview |
This recombinant human DHFR protein includes amino acids 1-187aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P00374 |
| Recommended Name |
Dihydrofolate reductase |
| Gene Name |
DHFR |
| Synonyms |
Dihydrofolate reductase |
| Species |
Human |
| Predicted Molecular Mass |
23.6 kDa |
| Expression System |
E.coli |
| Expression Range |
1-187aa |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Cell Biology, Signal Transduction |
| Target Function |
Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2. |
| Subcellular Location |
Mitochondrion. Cytoplasm. |
| Protein Family |
Dihydrofolate reductase family |
| Associated Diseases |
Megaloblastic anemia due to dihydrofolate reductase deficiency (DHFRD) |
| Tissue Specificity |
Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood. Expression is higher in the adult brain than in the fetal brain. |
