| Product Name |
Recombinant Human Defensin / Beta-defensin 3 / DEFB103 (DEFB103A) Protein |
| Product Overview |
This recombinant human Defensin / Beta-defensin 3 / DEFB103 (DEFB103A) protein includes amino acids 23-67aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P81534 |
| Recommended Name |
Beta-defensin 103 |
| Gene Name |
DEFB103A |
| Synonyms |
BD-3;DEFB-3;DEFB103;DEFB3;hBD-3;HBD3;HBP-3;HBP3 |
| Species |
Human |
| Predicted Molecular Mass |
7.3 kDa |
| Expression System |
E.coli |
| Expression Range |
23-67aa |
| Tag |
N-His |
| Purity |
>95% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Microbiology |
| Target Function |
Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistant E.faecium. No significant hemolytic activity was observed. |
| Subcellular Location |
Secreted. |
| Protein Family |
Beta-defensin family |
| Tissue Specificity |
Highly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. Low expression in salivary gland, bone marrow, colon, stomach, polyp and larynx. No expression in small intestine. |
