| Product Name |
Recombinant Human D2-Hydroxyglutarate Dehydrogenase (D2HGDH) Protein |
| Product Overview |
This recombinant human D2-Hydroxyglutarate Dehydrogenase (D2HGDH) protein includes amino acids 56-232 of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBS pH 7.4, 0.01% SKL, 5% Trehalose, 1% Mannitolprior to lyophilization. |
| Target Uniprot Id |
Q8N465 |
| Recommended Name |
D-2-hydroxyglutarate dehydrogenase, mitochondrial |
| Gene Name |
D2HGDH |
| Synonyms |
D2HGD |
| Species |
Human |
| Predicted Molecular Mass |
23kDa |
| Expression System |
E.coli |
| Expression Range |
56-232 |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
PBS pH 7.4, 0.01% SKL, 5% Trehalose, 1% Mannitol |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Metabolism |
| Target Function |
Catalyzes the oxidation of D-2-hydroxyglutarate (D-2-HG) to alpha-ketoglutarate. Also catalyzes the oxidation of other D-2-hydroxyacids, such as D-malate (D-MAL) and D-lactate (D-LAC). Exhibits high activities towards D-2-HG and D-MAL but a very weak activity towards D-LAC. |
| Subcellular Location |
Mitochondrion. |
| Protein Family |
FAD-binding oxidoreductase/transferase type 4 family |
| Associated Diseases |
D-2-hydroxyglutaric aciduria 1 (D2HGA1) |
