| Product Name |
Recombinant Human Cytochrome P450 26B1 (CYP26B1) Protein |
| Product Overview |
This recombinant human Cytochrome P450 26B1 (CYP26B1) protein includes amino acids 1-512 of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBS pH 7.4, 0.01% SKL, 5% Trehalose, 1% Mannitolprior to lyophilization. |
| Target Uniprot Id |
Q9NR63 |
| Recommended Name |
Cytochrome P450 26B1 |
| Gene Name |
CYP26B1 |
| Synonyms |
CYP26A2; P450RAI-2; Cytochrome P450 Family 26 Subfamily B Polypeptide 1; Cytochrome P450 retinoic acid-inactivating 2; Retinoic acid-metabolizing cytochrome |
| Species |
Human |
| Predicted Molecular Mass |
61kDa |
| Expression System |
E.coli |
| Expression Range |
1-512 |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
PBS pH 7.4, 0.01% SKL, 5% Trehalose, 1% Mannitol |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Cardiovascular |
| Target Function |
Involved in the metabolism of retinoic acid (RA), rendering this classical morphogen inactive through oxidation. Involved in the specific inactivation of all-trans-retinoic acid (all-trans-RA), with a preference for the following substrates: all-trans-RA > 9-cis-RA > 13-cis-RA. Generates several hydroxylated forms of RA, including 4-OH-RA, 4-oxo-RA, and 18-OH-RA. Catalyzes the hydroxylation of carbon hydrogen bonds of atRA primarily at C-4. Essential for postnatal survival. Plays a central role in germ cell development: acts by degrading RA in the developing testis, preventing STRA8 expression, thereby leading to delay of meiosis. Required for the maintenance of the undifferentiated state of male germ cells during embryonic development in Sertoli cells, inducing arrest in G0 phase of the cell cycle and preventing meiotic entry. Plays a role in skeletal development, both at the level of patterning and in the ossification of bone and the establishment of some synovial joints.; Has also a significant activity in oxidation of tazarotenic acid and may therefore metabolize that xenobiotic in vivo. |
| Subcellular Location |
Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. |
| Protein Family |
Cytochrome P450 family |
| Associated Diseases |
Radiohumeral fusions with other skeletal and craniofacial anomalies (RHFCA) |
| Tissue Specificity |
Highly expressed in brain, particularly in the cerebellum and pons. |
