| Product Name |
Recombinant Human Cyclin D2 (CCND2) Protein |
| Product Overview |
This recombinant human Cyclin D2 (CCND2) protein includes amino acids 68-278aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P30279 |
| Recommended Name |
G1/S-specific cyclin-D2 |
| Gene Name |
CCND2 |
| Synonyms |
Cyclin D2, KIAK0002, MGC102758. |
| Species |
Human |
| Predicted Molecular Mass |
27 kDa |
| Expression System |
E.coli |
| Expression Range |
68-278aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Cell Biology |
| Target Function |
Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. |
| Subcellular Location |
Nucleus. Cytoplasm. Nucleus membrane.; [Isoform 2]: Cytoplasm. |
| Protein Family |
Cyclin family, Cyclin D subfamily |
| Associated Diseases |
Megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 3 (MPPH3) |
