| Product Name |
Recombinant Human Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) Protein |
| Product Overview |
This recombinant human Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) protein includes amino acids 52-211aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P16410 |
| Recommended Name |
Cytotoxic T-lymphocyte protein 4 |
| Gene Name |
CTLA4 |
| Synonyms |
Cytotoxic T-lymphocyte protein 4 isoform CTLA4-TM precursor , CD, CD152, CELIAC3, GRD4, GSE, ICOS, I |
| Species |
Human |
| Predicted Molecular Mass |
22 kDa |
| Expression System |
E.coli |
| Expression Range |
52-211aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Cancer, Immunology |
| Target Function |
Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. |
| Subcellular Location |
Cell membrane; Single-pass type I membrane protein. Note=Exists primarily an intracellular antigen whose surface expression is tightly regulated by restricted trafficking to the cell surface and rapid internalization. |
| Associated Diseases |
Systemic lupus erythematosus (SLE); Diabetes mellitus, insulin-dependent, 12 (IDDM12); Celiac disease 3 (CELIAC3); Autoimmune lymphoproliferative syndrome 5 (ALPS5) |
| Tissue Specificity |
Widely expressed with highest levels in lymphoid tissues. Detected in activated T-cells where expression levels are 30- to 50-fold less than CD28, the stimulatory coreceptor, on the cell surface following activation. |
