| Product Name |
Recombinant Human CASP2 And RIPK1 Domain Containing Adaptor With Death Domain (CRADD) Protein |
| Product Overview |
This recombinant human CASP2 And RIPK1 Domain Containing Adaptor With Death Domain (CRADD) protein includes amino acids 1-140aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
P78560 |
| Recommended Name |
Death domain-containing protein CRADD |
| Gene Name |
CRADD |
| Synonyms |
Caspase and RIP adapter with death domain, RAIDD |
| Species |
Human |
| Predicted Molecular Mass |
16 kDa |
| Expression System |
E.coli |
| Expression Range |
1-140aa |
| Tag |
N-6His |
| Purity |
>95% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Apoptosis |
| Target Function |
Adapter protein that associates with PIDD1 and the caspase CASP2 to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis. Also recruits CASP2 to the TNFR-1 signaling complex through its interaction with RIPK1 and TRADD and may play a role in the tumor necrosis factor-mediated signaling pathway. |
| Subcellular Location |
Cytoplasm. Nucleus. |
| Associated Diseases |
Mental retardation, autosomal recessive 34, with variant lissencephaly (MRT34) |
| Tissue Specificity |
Constitutively expressed in most tissues, with particularly high expression in adult heart, testis, liver, skeletal muscle, fetal liver and kidney. |
