| Product Name |
Recombinant Human Collagen Type IV Alpha 1 (COL4a1) Protein |
| Product Overview |
This recombinant human Collagen Type IV Alpha 1 (COL4a1) protein includes amino acids 1444-1669aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBSprior to lyophilization. |
| Target Uniprot Id |
P02462 |
| Recommended Name |
Collagen alpha-1(IV) chain |
| Gene Name |
COL4A1 |
| Synonyms |
COL4-A1; Arresten; Collagen Alpha-1(IV)chain |
| Species |
Human |
| Predicted Molecular Mass |
31 kDa |
| Expression System |
E.coli |
| Expression Range |
1444-1669aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
PBS |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Cancer |
| Target Function |
Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.; Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. |
| Subcellular Location |
Secreted, extracellular space, extracellular matrix, basement membrane. |
| Protein Family |
Type IV collagen family |
| Associated Diseases |
Brain small vessel disease with or without ocular anomalies (BSVD); Hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC); Porencephaly 1 (POREN1); Intracerebral hemorrhage (ICH); Tortuosity of retinal arteries (RATOR); Schizencephaly (SCHZC) |
| Tissue Specificity |
Highly expressed in placenta. |
