| Product Name |
Recombinant Human Collagen Type XII (COL12/COL12A1) Protein |
| Product Overview |
This recombinant human Collagen Type XII (COL12/COL12A1) protein includes amino acids 2802-3063aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBSprior to lyophilization. |
| Target Uniprot Id |
Q99715 |
| Recommended Name |
Collagen alpha-1(XII) chain |
| Gene Name |
COL12A1 |
| Synonyms |
COL12-A1; COL12A1L; COL12A1; Collagen Alpha-1(XII)chain |
| Species |
Human |
| Predicted Molecular Mass |
33/70 kDa |
| Expression System |
E.coli |
| Expression Range |
2802-3063aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
PBS |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Signal Transduction |
| Target Function |
Type XII collagen interacts with type I collagen-containing fibrils, the COL1 domain could be associated with the surface of the fibrils, and the COL2 and NC3 domains may be localized in the perifibrillar matrix. |
| Subcellular Location |
Secreted, extracellular space, extracellular matrix. |
| Protein Family |
Fibril-associated collagens with interrupted helices (FACIT) family |
| Associated Diseases |
Ullrich congenital muscular dystrophy 2 (UCMD2); Bethlem myopathy 2 (BTHLM2) |
| Tissue Specificity |
Found in collagen I-containing tissues: both isoform 1 and isoform 2 appear in amnion, chorion, skeletal muscle, small intestine, and in cell culture of dermal fibroblasts, keratinocytes and endothelial cells. Only isoform 2 is found in lung, placenta, ki |
