| Product Name |
Recombinant Human Coactosin Like 1 (COTL1) Protein |
| Product Overview |
This recombinant human Coactosin Like 1 (COTL1) protein includes amino acids 1-142aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id |
Q14019 |
| Recommended Name |
Coactosin-like protein |
| Gene Name |
COTL1 |
| Synonyms |
Coactosin-like protein, CLP, FLJ43657, MGC19733. |
| Species |
Human |
| Predicted Molecular Mass |
19 kDa |
| Expression System |
E.coli |
| Expression Range |
1-142aa |
| Tag |
N-6His |
| Purity |
>95% |
| Formulation |
Lyophilized |
| Buffer |
Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Signal Transduction |
| Target Function |
Binds to F-actin in a calcium-independent manner. Has no direct effect on actin depolymerization. Acts as a chaperone for ALOX5 (5LO), influencing both its stability and activity in leukotrienes synthesis. |
| Subcellular Location |
Cytoplasm. Cytoplasm, cytoskeleton. Nucleus. |
| Protein Family |
Actin-binding proteins ADF family, Coactosin subfamily |
| Tissue Specificity |
Widely expressed with highest levels in placenta, lung, kidney and peripheral blood leukocytes and lower levels in brain, liver and pancreas. |
