| Product Name |
Recombinant Human Claudin 1 (CLDN1) Protein |
| Product Overview |
This recombinant human Claudin 1 (CLDN1) protein includes amino acids 50-197aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBSprior to lyophilization. |
| Target Uniprot Id |
O95832 |
| Recommended Name |
Claudin-1 |
| Gene Name |
CLDN1 |
| Synonyms |
CLD1; ILVASC; SEMP1; Senescence-Associated Epithelial Membrane Protein 1 |
| Species |
Human |
| Predicted Molecular Mass |
46 kDa |
| Expression System |
E.coli |
| Expression Range |
50-197aa |
| Tag |
N-6His |
| Purity |
>90% |
| Formulation |
Lyophilized |
| Buffer |
PBS |
| Storage Condition |
1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction |
Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications |
Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area |
Signal Transduction |
| Target Function |
Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions.; (Microbial infection) Acts as a co-receptor for hepatitis C virus (HCV) in hepatocytes. Associates with CD81 and the CLDN1-CD81 receptor complex is essential for HCV entry into host cell. Acts as a receptor for dengue virus. |
| Subcellular Location |
Cell junction, tight junction. Cell membrane; Multi-pass membrane protein. Basolateral cell membrane. |
| Protein Family |
Claudin family |
| Associated Diseases |
Ichthyosis-sclerosing cholangitis neonatal syndrome (NISCH) |
| Tissue Specificity |
Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis. |
